Regulatory

Part:BBa_K4205002:Design

Designed by: Xinyuan Wang   Group: iGEM22_NJMU-China   (2022-09-04)


PpbrA-PmerR


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 58
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 58
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 58
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 58
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We put a sequence(TACTAG) between the two promoters to prevent spatial interference between them.


Source

The composite element consists of BBa_K346002 and BBa_K346054 in series.

References